Products

TOOLS Hotstart Polymerase a monoclonal antibody which can inhibit Taq DNA polymerase activity when bound to it. Due to its unique thermo stability and high affinity to Taq DNA polymerase, TOOLS Hotstart Polymerase Antibody blocks Taq DNA polymerase activity at temperatures up to 65°C but releases fully active polymerase after heat treatment at 95°C for 30 seconds. TOOLS Hotstart Polymerase DNA Polymerase is recombinant Taq DNA polymerase contained TOOLS Hotstart Polymerase antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 95°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR, which provide increased sensitivity, specificity, and yield, while allowing assembly of reactions at room temperature. The use of this antibody helps reduce PCR optimization requirements, reaction set-up time and effort, handling of reaction components, and contamination risk. No modifications to PCR reactions or protocols are necessary. This product has a 5’→3’ DNA polymerase activity and a 5’→3’ exonuclease activity, and lacks a 3’→5’ proofreading function. The PCR products contain A at the 3'-end and can be directly cloned into TA-Vectors.